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GEPPO SARTORI

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Position

Ricercatore Universitario a tempo indeterminato

Structure

Dipartimento di Scienze Biomediche - DSB

Address

VIALE G. COLOMBO, 3 - PADOVA

Telephone

0498276141

E-mail

geppo.sartori@unipd.it

Curriculum vitae in brief
(for a detailed CV, see the attached pdf file)

Geppo Sartori, born on May 16, 1966, is a Researcher of Molecular Biology at the Department of Biomedical Sciences of the University of Padova since february 2001. In the year 1992 he obtained the degree in Biological Sciences at the University of Padova by discussing a thesis concerning his contribute to the project aimed to obtain the first complete eukaryotic genome sequence, that of the yeast Saccharomyces cerevisiae.
In 1997 he obtained the PhD in Biochemistry and Biophysics at the University of Padova by developing a research project focused on the functional analysis of an yeast protein belonging to the family of haemoglobins but characterized by an unusual structure. His work is a contribution to the understanding of the role played by haemoglobins in the unicellular organisms.
After the PhD degree, he obtained a post-doctoral fellowship of the University of Padova and he continued his work, partly in the J. Verdière laboratory at the Centre de Génétique Moléculaire of C.N.R.S. (Gif sur Yvette, France), to study in detail some aspects of gene transcription regulation by oxygen in yeast.
When he returned to Italy in 1997, he started the functional characterization of some unknown, evolutionarily conserved genes identified during the yeast genome sequencing. The study, in particular, of the two BUD32 and KAE1 genes and of their interactors lead to the discovery of the KEOPS/EKC multiprotein complex, involved in transcription regulation and telomere homeostasis.
Starting from 2008, he started a collaboration with Prof. Leonardo Salviati (Dept. of Woman and Child Health, University of Padova) and participated in several projects aimed to the development of yeast models for the biochemical and functional characterization of mutations in human genes related to rare mitochondrial and metabolic genetic diseases. A similar approach, thanks to a collaboration with Prof. Paolo Bernardi at the Department of Biomedical Sciences, has also been used to study the role played by ATP-synthase in the formation of the Permeability Transition Pore (PTP), a channel of the inner mitochondrial membrane playing a key role in several physio-pathological mechanisms, but whose molecular identity is still under study.
Finally, in the last three years his main research interest was focused on the study of the low complexity domains of the proteins and in their involvement in complex protein-protein interaction networks, such as those centered on hub proteins that, like the VHL oncosuppressor, play a key role in cell proliferation control.

Notices

Office hours

  • Wednesday from 11:00 to 13:00
    at Laboratorio 54 dell'area scientifica, V piano nord, complesso interdipartimentale Vallisneri

  • Tuesday from 12:00 to 13:00
    at Laboratorio 54 dell'area scientifica, V piano nord, complesso interdipartimentale Vallisneri

  • Thursday from 12:00 to 13:00
    at Laboratorio 54 dell'area scientifica, V piano nord, complesso interdipartimentale Vallisneri

  • Monday from 11:00 to 12:00
    at Laboratorio 54 dell'area scientifica, V piano nord, complesso interdipartimentale Vallisneri

Teachings

Publications

Publication list of the last 5 years (for a complete list, see the attached pdf file)

1. Cerqua C, Morbidoni V, Desbats MA, Doimo M, Frasson C, Sacconi S, Baldoin MC, Sartori G, Basso G, Salviati L, Trevisson E (2018). COX16 is required for assembly of cytochrome c oxidase in human cells and is involved in copper delivery to COX2. Biochim. Biophys. Acta Bioenerg. 1859: 244-252;

2. Fonseca LV, Doimo M, Calderan C, Desbats MA, Acosta MJ, Cerqua C, Cassina M, Ashraf S, Hildebrandt F, Sartori G, Navas P, Trevisson E, Salviati L (2018). Mutations in COQ8B (ADCK4) found in patients with steroid-resistant nephrotic syndrome alter COQ8B function. Hum Mutat 39: 406-414;

3. Panfili M, Sartori G, Lanzellotti D, Martin M, Padrini R (2017). Unduly enhanced response to tolvaptan in a woman showing syndrome of inappropriate antidiuretic hormone secretion. An investigation of possible causes. AACE Clinical Case Rep 3: 357-360;

4. Minervini G, Lopreiato R, Bortolotto R, Falconieri A, Sartori G, Tosatto SCE (2017). Novel interactions of the von Hippel-Lindau (pVHL) tumor suppressor with the CDKN1 family of cell cycle inhibitors. Sci Rep 7: 46562;

5. Vetro A, Savasta S, Russo Raucci A, Cerqua C, Sartori G, Limongelli I, Forlino A, Maruelli S, Perucca P, Vergani D, Mazzini G, Mattevi A, Stivala LA, Salviati L, Zuffardi O (2017). MCM5: a new actor in the link between DNA replication and Meier-Gorlin syndrome. Eur J Hum Genet 25: 646-50;

6. Magro M, Baratella D, Jakubec P, Zoppellaro G, Tucek J, Aparicio C, Venerando R, Sartori G, Francescato F, Mion F, Gabellini N, Zboril R, Vianello F (2015). Triggering Mechanism for DNA Electrical Conductivity: Reversible Electron Transfer between DNA and Iron Oxide Nanoparticles. Adv Funct Mater 25: 1822-31;

7. Carraro M, Giorgio V, Šileikyté J, Sartori G, Forte M, Lippe G, Zoratti M, Szabò I, Bernardi P (2014).Channel formation by yeast F-ATP synthase and the role of dimerization in the mitochondrial permeability transition. J Biol Chem 289: 15980-5;

8. Doimo M, Desbats MA, Baldoin MC, Lenzini E, Basso G, Murphy E, Graziano C, Seri M, Burlina A, Sartori G, Trevisson E, Salviati L (2013).Functional analysis of missense mutations of OAT, causing gyrate atrophy of choroid and retina. Hum Mutat 34: 229-36;

9. Cassandrini D, Cilio MR, Bianchi M, Doimo M, Balestri M, Tessa A, Rizza T, Sartori G, Meschini MC, Nesti C, Tozzi G, Petruzzella V, Piemonte F, Bisceglia L, Bruno C, Dionisi-Vici C, D'Amico A, Fattori F, Carrozzo R, Salviati L, Santorelli FM, Bertini E (2013). Pontocerebellar hypoplasia type 6 caused by mutations in RARS2: definition of the clinical spectrum and molecular findings in five patients. J Inherit Metab Dis 36: 43-53;

Research Area

The research work is aimed at the study of the protein-protein interactions and, specifically, at the identification and characterization of the role played by the low-complexity domains in the complex interaction networks centered on some proteins playing a central role in the onset of some tumor and neurodegenerative diseases.
For this purpose, yeast models has been constructed in order to study, by the yeast two-hybrid technique and others genetic and biochemical approaches, new interactors of the VHL (von Hippel-Lindau) tumor suppressor and of some proteins (in particular TDP43 and C9Orf72)involved in the onset of ALS (Amyotrophic Lateral Sclerosis), a neurodegenerative disease mainly affecting motor neurons.

Thesis proposals

The proposed projects are focused on the research fields described in the specific section (Aree di ricerca).
From a methodological point of view, the students will have the opportunity to gain a direct experience in the use of the principal modern methods of the recombinant DNA technology (nucleic acid cloning and manipulation) and in the production and analysis of recombinant proteins in bacteria.
It will be also possible for the student to take part in the setup of mammalian cell cultures (cell lines and neuron primary cultures) and in the analysis of the intracellular localization of GFP-tagged proteins.
Moreover, it will be possible to gain a specific expertise in the use of the S. cerevisiae yeast, a particularly powerful and versatile eukaryotic model both in the genetic and molecular biology field.